Journal: PLoS ONE
Article Title: Protein Expression of ZEB2 in Renal Cell Carcinoma and Its Prognostic Significance in Patient Survival
doi: 10.1371/journal.pone.0062558
Figure Lengend Snippet: (A) Up-regulated expression of the ZEB2 protein was observed in 8/10 RCC cases using western blot when compared to the adjacent non-malignant renal tissues. T, RCC tissue; N, non-neoplastic renal tissue. (B) High ZEB2 expression was observed in an RCC sample (case 63), in which more than 90% of the tumor cells revealed positive immunostaining of ZEB2 in the cytoplasm and nuclei ( upper panel , ×100). (C) A RCC case (case 56) demonstrated low ZEB2 expression, in which fewer than 50% of the tumor cells showed immunoreactivity of the ZEB2 protein ( upper panel , ×100). (D) Nearly negative expression of the ZEB2 protein was demonstrated in an RCC case (case 72, upper panel , ×100). The lower panels indicate the higher magnification (×400) from the area of the boxes in (B), (C) and (D), respectively.
Article Snippet: The slides were incubated with a rabbit polyclonal anti-ZEB2 (Cat. No. HPA003456, Sigma, St. Louis, MO, USA, 1∶100 dilution), anti-E-cadherin (Dako, Glostrup, Denmark, 1∶50 dilution) and anti-vimentin antibodies (Dako, Glostrup, Denmark, 1∶200 dilution) overnight at 4°C in a humidified chamber.
Techniques: Expressing, Western Blot, Immunostaining